Enzymatic grafting of chitosan onto Bombyx mori silk fibroin: kinetic and IR vibrational studies.

@article{Sampaio2005EnzymaticGO,
  title={Enzymatic grafting of chitosan onto Bombyx mori silk fibroin: kinetic and IR vibrational studies.},
  author={Sandra de F. Mendes Sampaio and Paola Taddei and Patrizia Monti and Johanna Buchert and Giuliano Freddi},
  journal={Journal of biotechnology},
  year={2005},
  volume={116 1},
  pages={21-33}
}
The potential for using tyrosinase to graft the polysaccharide chitosan (Ch) onto Bombyx mori silk fibroin (SF) was examined. FT-IR spectroscopy coupled to HPLC amino acid analysis showed that mushroom tyrosinase (MT) catalyses the oxidation of tyrosine (Tyr) of SF to electrophilic o-quinones. Kinetic studies showed that only a fraction of the Tyr residues available on the SF chain were oxidized. This result was interpreted in the light of the structure assumed by SF in aqueous solution: Tyr… CONTINUE READING
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