Enzymatic generation of ceramide induces membrane restructuring: Correlated AFM and fluorescence imaging of supported bilayers.

@article{Ira2009EnzymaticGO,
  title={Enzymatic generation of ceramide induces membrane restructuring: Correlated AFM and fluorescence imaging of supported bilayers.},
  author={Ira and Shan Shan Zou and Daniel M. Carter Ramirez and Sapphire Vanderlip and William W. Ottawa Ogilvie and Zygmunt J. Jakubek and Linda J Johnston},
  journal={Journal of structural biology},
  year={2009},
  volume={168 1},
  pages={
          78-89
        }
}
The effect of enzymatic generation of ceramide on phase separated bilayers with a mixture of co-existing fluid and liquid-ordered phases has been examined using a combination of atomic force microscopy (AFM) and fluorescence imaging. Supported lipid bilayers prepared from a DOPC/sphingomyelin/cholesterol mixture were imaged prior to, during and after incubation with sphingomyelinase by total internal reflection fluorescence (TIRF) microscopy. Enzyme treatment resulted in the growth of large dye… CONTINUE READING
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