Enzymatic formation of biparental figure-eight molecules from plasmid DNA and their resolution in E. coli.

@article{West1983EnzymaticFO,
  title={Enzymatic formation of biparental figure-eight molecules from plasmid DNA and their resolution in E. coli.},
  author={Stephen C West and Jill K. Countryman and Paul Howard-Flanders},
  journal={Cell},
  year={1983},
  volume={32 3},
  pages={817-29}
}
A key intermediate in general genetic recombination is a structure in which two double-stranded DNA molecules are covalently linked by a single-strand crossover characteristic of a Holliday junction. When the DNA molecules are circular, the recombinant structures take the form of a figure eight. We have used purified E. coli enzymes to construct biparental figure-eight DNA molecules in vitro from the DNA of two partially homologous plasmids. When purified figure-eight structures are transfected… CONTINUE READING

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