Enzymatic dissociation, flow cytometric analysis, and culture of normal mouse mammary tissue.

@article{Prater2013EnzymaticDF,
  title={Enzymatic dissociation, flow cytometric analysis, and culture of normal mouse mammary tissue.},
  author={Michael D. Prater and Mona A H Shehata and Christine J Watson and John Stingl},
  journal={Methods in molecular biology},
  year={2013},
  volume={946},
  pages={
          395-409
        }
}
Evidence is emerging that the mouse mammary epithelium is arranged as a hierarchy that spans from stem cells to lineage-restricted progenitor cells to differentiated luminal and myoepithelial cells. The use of fluorescence-activated cell sorting (FACS) in combination with quantitative functional clonal assays represents a powerful tool for studying the properties of mouse mammary stem and progenitor cells. This chapter outlines the experimental procedures for generating single viable cell… CONTINUE READING
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