Enzymatic determination of subpicomole quantities of phospho-L-arginine in nerve cytoplasm.

Abstract

An ultramicro phospho-L-arginine (Arg-P) assay for nanoliter-volume biological samples is described. The assay and method is demonstrated to be reliable and reproducible for the detection of subpicomole quantities of both Arg-P and ATP in serial samples of cytoplasm from a single, isolated giant nerve fiber from the crayfish Procambarus clarkii. The assay… (More)

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