Enzymatic detection of protein translocation

@article{Wehrman2005EnzymaticDO,
  title={Enzymatic detection of protein translocation},
  author={Tom S Wehrman and Clayton L. Casipit and Nevin M Gewertz and Helen M. Blau},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={521-527}
}
Fundamental to eukaryotic cell signaling is the regulation of protein function by directed localization. Detection of these events has been largely qualitative owing to the limitations of existing technologies. Here we describe a method for quantitatively assessing protein translocation using proximity-induced enzyme complementation. The complementation assay for protein translocation (CAPT) is derived from β-galactosidase and comprises one enzyme fragment, ω, which is localized to a particular… CONTINUE READING
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SHOWING 1-10 OF 38 REFERENCES

In vivo imaging of b-galactosidase activity using far red fluorescent switch

  • Tung, C.H
  • Cancer Res
  • 2004

Development of a dual luciferase reporter screening assay for the detection of synthetic glucocorticoids in animal tissues

  • Schumacher, S.B
  • Analyst 128,
  • 2003

NF-kB/IkB pathway signaling

  • Zhao, X. et al. Homogeneous assays for cellular protein deg complementation
  • Assay Drug Dev. Technol. 1, 823–833
  • 2003

Epidermal growth factor receptor dimerization monitored in live cells

  • Blakely, B.T
  • Nat. Biotechnol
  • 2000

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