Enzymatic conversion of dethiobiotin to biotin in cell-free extracts of a Bacillus sphaericus bioB transformant.

@article{Ohshiro1994EnzymaticCO,
  title={Enzymatic conversion of dethiobiotin to biotin in cell-free extracts of a Bacillus sphaericus bioB transformant.},
  author={Takashi Ohshiro and Masahiro Yamamoto and Yoshikazu Izumi and Bernadette Tse Sum Bui and Dominique Florentin and Andr{\'e}e Marquet},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1994},
  volume={58 9},
  pages={1738-41}
}
The activity of biotin synthase, responsible for biotin synthesis from dethiobiotin, was demonstrated in a completely defined reaction mixture with cell-free extracts of a Bacillus sphaericus bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine was active, while L-methionine and L-cysteine had no significant effect. Protein concentrations higher than 15 mg/ml in the reaction mixture were needed to detect biotin synthase activity. When dialyzed cell-free extracts… CONTINUE READING