Enzymatic cascade based fluorescent DNAzyme machines for the ultrasensitive detection of Cu(II) ions.


A novel enzymatic cascade based fluorescent DNAzyme machine has been developed for the amplified detection of copper (Cu(2+)) ions. This is the first attempt to carry out the combination of the self-cleaving DNAzyme and the polymerase/endonuclease reaction cycles involving cleaved substrate extension. In the presence of Cu(2+) ions, the enzyme strand… (More)
DOI: 10.1016/j.bios.2014.03.065