Enzymatic assays for native plasmin, plasminogen and plasminogen activators in bovine milk.

@article{SaintDenis2001EnzymaticAF,
  title={Enzymatic assays for native plasmin, plasminogen and plasminogen activators in bovine milk.},
  author={Bernard Saint-Denis and G{\'e}rard Humbert and Jean Luc Gaillard},
  journal={The Journal of dairy research},
  year={2001},
  volume={68 3},
  pages={
          437-49
        }
}
Rapid and sensitive assays for plasmin, plasminogen and plasminogen activators (PA) were developed and applied to bovine milk. The reaction medium was clarified by addition of a dissolving agent after hydrolysis of a fluorescent substrate specific for plasmin. This final step enabled the use of larger sample amount with higher substrate concentration than other methods, and avoided previous sample preparation. The use of 4 g gelatin/l in buffers preserved plasmin activity, thus avoiding risks… CONTINUE READING

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