Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes.

@article{Belda1982EnzymaticAO,
  title={Enzymatic assays: optimization of systems by using pyruvate kinase and lactate dehydrogenase as auxiliary enzymes.},
  author={Fern{\'a}ndez Belda and F G Carmona and François Canovas and Juan C. G{\'o}mez and Jos{\'e} A. Lozano},
  journal={Revista espanola de fisiologia},
  year={1982},
  volume={38 3},
  pages={327-32}
}
A method to optimize enzymatic assays by using pyruvate kinase and lactate dehydrogenase enzymes is presented and applied to mitochondrial ATPase as an example. Optimum amounts of auxiliary enzymes, to obtain either a 99% of the initial rate in a given time (t99) or a given lag period (L), are calculated from their apparent Michaelis constants (Kapp) in the medium used and their prices per enzymatic international unit. 

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