Enzymatic assay of allantoin in serum using allantoinase and allantoate amidohydrolase.

@article{Muratsubaki2006EnzymaticAO,
  title={Enzymatic assay of allantoin in serum using allantoinase and allantoate amidohydrolase.},
  author={Haruhiro Muratsubaki and Kaoru Satake and Keiichiro Enomoto},
  journal={Analytical biochemistry},
  year={2006},
  volume={359 2},
  pages={161-6}
}
A new enzymatic assay for specifically measuring allantoin concentration in serum has been developed. The currently used methods for allantoin analysis are time consuming and nonspecific or depend on the use of expensive equipment. In our method, allantoin is converted to allantoate by the action of allantoinase (EC 3.5.2.5). The allantoate produced is hydrolyzed to ureidoglycine and ammonia by the action of allantoate amidohydrolase (EC 3.5.3.9). Nicotinamide adenine dinucleotide phosphate… CONTINUE READING