Enzymatic and regulatory attributes of trehalose-6-phosphate phosphatase from Candida utilis and its role during thermal stress.

@article{Lahiri2014EnzymaticAR,
  title={Enzymatic and regulatory attributes of trehalose-6-phosphate phosphatase from Candida utilis and its role during thermal stress.},
  author={Sagar Lahiri and Shakri Banerjee and Trina Dutta and Shinjinee Sengupta and Sandip Dey and Rusha Roy and Devlina Sengupta and Krishnananda Chattopadhyay and Anil Kumar Ghosh},
  journal={Journal of cellular physiology},
  year={2014},
  volume={229 9},
  pages={1245-55}
}
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis, showed significant homology with known TPP sequences from related organisms. The full length gene sequence of TPP of C. utilis was identified using rapid amplification of cDNA ends-PCR reaction (RACE… CONTINUE READING
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