Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase.

@article{Weser1995EnzymaticAI,
  title={Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase.},
  author={Ulrich Weser and Hedwig Etsp{\"u}ler and Yoka Kaup},
  journal={FEBS letters},
  year={1995},
  volume={375 3},
  pages={280-2}
}
Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 +/- 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 +/- 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal… CONTINUE READING