The objective of this study was to assess the enzymatic and non-enzymatic antioxidant levels in patients with iron-deficiency anemia (IDA). Forty patients with IDA and forty healthy individuals with their informed consent were selected for the study. The enzymatic antioxidantscatalase, glutathione peroxidase and superoxide dismutase; non-enzymatic antioxidants-vitamin C and E and total antioxidant activity (TAA) were also analyzed. The lipid peroxidation parameter thiobarbituric acid reactive substance was analyzed. The enzymatically antioxidants were found to be statistically increased but vitamin C and E and TAA were significantly lowered. Diminished antioxidant status disturbed oxidant-antioxidant balance alleviating oxidative stress state. KeywordsAntioxidant, catalase, enzymatic, glutathione peroxidase, iron deficiency anemia.