Enzymatic analyses in organic solvents.

Abstract

Horseradish peroxidase has been found to vigorously act as a catalyst in a number of water-immiscible organic solvents. The rates of peroxidase-catalyzed oxidation of p-anisidine with H(2)O(2) in toluene, benzene, ethyl and butyl acetates, and ether are in the range of 10-25% of that in water (pH 7.0) at the same reactant concentrations. Per oxidase was… (More)

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