Corpus ID: 33216289

Enzymatic addition of O-GlcNAc to nuclear and cytoplasmic proteins. Identification of a uridine diphospho-N-acetylglucosamine:peptide beta-N-acetylglucosaminyltransferase.

@article{Haltiwanger1990EnzymaticAO,
  title={Enzymatic addition of O-GlcNAc to nuclear and cytoplasmic proteins. Identification of a uridine diphospho-N-acetylglucosamine:peptide beta-N-acetylglucosaminyltransferase.},
  author={R. Haltiwanger and G. Holt and G. Hart},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 5},
  pages={
          2563-8
        }
}
  • R. Haltiwanger, G. Holt, G. Hart
  • Published 1990
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • An assay for the enzyme responsible for the addition of O-linked N-acetylglucosamine (O-GlcNAc) to proteins, a UDP-N-acetylglucosamine:peptide N-acetylglucosaminyltransferase, is reported using the synthetic peptide YSDSPSTST as the acceptor substrate. The activity is linearly dependent on time, enzyme, and substrate concentration. Replacement of the proline with a glycine in the peptide renders it ineffective as a substrate, whereas changing of the aspartic acid to a glycine has no effect… CONTINUE READING
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