Enzymatic activity assay of D-hydantoinase by isothermal titration calorimetry. Determination of the thermodynamic activation parameters for the hydrolysis of several substrates.

@article{AndjarSnchez2006EnzymaticAA,
  title={Enzymatic activity assay of D-hydantoinase by isothermal titration calorimetry. Determination of the thermodynamic activation parameters for the hydrolysis of several substrates.},
  author={Montserrat And{\'u}jar-S{\'a}nchez and Francisco Javier Las Heras-V{\'a}zquez and Josefa Mar{\'i}a Clemente-Jim{\'e}nez and S Mart{\'i}nez-Rodr{\'i}guez and Ana C{\'a}mara-Artigas and Felipe Rodr{\'i}guez-Vico and Vicente Jara-P{\'e}rez},
  journal={Journal of biochemical and biophysical methods},
  year={2006},
  volume={67 1},
  pages={57-66}
}
Isothermal titration calorimetry (ITC) has been applied to the determination of the activity of D-hydantoinase (EC 3.5.2.2) with several substrates by monitoring the heat released during the reaction. The method is based on the proportionality between the reaction rate and the thermal power (heat/time) generated. Microcalorimetric assays carried out at different temperatures provided the dependence of the catalytic rate constant on temperature. We show that ITC assay is a nondestructive method… CONTINUE READING