Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom

  title={Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom},
  author={Mitchel Otieno Okumu and James Mucunu Mbaria and Joseph Kangangi Gikunju and Paul Gichohi Mbuthia and Vincent Odongo Madadi and Francis Okumu Ochola},
  journal={BMC Research Notes},
Naja ashei is a snake of medical importance in Kenya, Ethiopia, Somalia, Uganda, and Tanzania. Little is known about the enzymatic (snake venom phospholipases A2; svPLA2’s) and toxic (lethal) activities of N. ashei venom and crucially, the safety and capacity of available antivenom to neutralize these effects. This study aimed to determine the enzymatic and toxic activities of N. ashei venom and the capacity of Indian and Mexican manufactured antivenoms to neutralize these effects. The protein… Expand
3 Citations
Artemia salina as an animal model for the preliminary evaluation of snake venom-induced toxicity
The findings suggest that the toxicity of the venoms in Artemia salina and the dermonecrotic bioassay in mice have a similar trend but differ from the lethality test in mice. Expand
Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity
The results show that the mechanism of protein–lipid interactions depends on both the presence of lipid polar parts (especially zwitterionic type of lipids) and the degree of membrane saturation (the greatest-for unsaturated lipids). Expand
Phytochemical Analysis and Investigation of the Antimicrobial and Cytotoxic Activities of Croton dichogamus Pax Crude Root Extracts
It is reported that C. dichogamus had the antimicrobial activity confirming the folklore claim and made a strong case for isolation of novel anticancer lead compounds. Expand


Crude catfish sting venom could be used to isolate structurally unique compounds of specific activites and is revealed to be high toxicity upon testing with erythrocytes and brine shrimp napulii. Expand
Aqueous Leaf Extract of Jatropha gossypiifolia L. (Euphorbiaceae) Inhibits Enzymatic and Biological Actions of Bothrops jararaca Snake Venom
The results demonstrate the potential antiophidic activity of J. gossypiifolia extract, including its significant action upon local effects, suggesting that it may be used as a new source of bioactive molecules against bothropic venom. Expand
Isolation and Identification of Cytotoxic and Biological Active Toxin from the Puffer Fish Arothron stellatus
It was revealed that the puffer fish toxin contains tetrodotoxin (TTX), related compounds 4-epi TTX and anhydro-TTX, which could be the potential candidate in the field of anticancer drug discovery against human cervical cancer cells. Expand
First Look at the Venom of Naja ashei
The main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2) and the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has been confirmed. Expand
Isolation and Characterization of Biologically Active Venom Protein from Sea Snake Enhydrina schistosa
The results suggested that the sea snake venom might be feasible sources for biologically active substances and this low molecular weight component of the venom protein could be used in potentially serve biological and pharmaceutical aspects. Expand
Honeybee venom: influence of collection on quality and cytotoxicity
EnglishApitoxin is the venom produced by bees. It is a complex chemical compound, rich in protein substances and with pharmacological effects. This study was carried out with the objective ofExpand
Enzymes in Snake Venom
A number of animal venoms including those of snakes, gila monsters, scorpions, spiders, and bees contain various enzymes in addition to toxic elements. These enzyme activities can not be ignored whenExpand
Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis
A new group of low abundant proteins containing the Ig-like domain that have not been previously described in snake venoms are identified, showing exceptional antimicrobial effects both alone and in combination with antibiotics. Expand
Bufadienolides from the Venom of Bufo Bufo gargarizans and Their Enzyme Inhibition Activities and Brine Shrimp Lethality
A new bufadienolide named dyscinobufotalin (1), along with twenty known bufadienolides (2–21) were isolated from the venom of Bufo bufo gargarizans. Their structures were elucidated by spectroscopicExpand
An investigation of the biological activity of bullrout (Notesthes robusta) venom.
  • S. Hahn, J. O’Connor
  • Biology, Medicine
  • Toxicon : official journal of the International Society on Toxinology
  • 2000
A single venom protein isolated from bullrout venom is attributed with causing pain in human subjects, and the sensations elicited by this novel algesic protein are consistent with chemical stimulation of polymodal nociceptors. Expand