Corpus ID: 211269262

Enzymatic Joining of Polynucleotides

  title={Enzymatic Joining of Polynucleotides},
  author={Zach W. Halli},
Polydeoxythymidylate in which the 5’-terminal phosphate is linked by a pyrophosphate bond to adenosine 5’-phosphate (poly dT-adenylate) serves as an intermediate in the reactions catalyzed by the Escherichia coli and T4-induced polynucleotide joining enzymes in the presence of polydeoxyadenylate. Adenosine 5’-phosphate release and phosphodiester bond formation occur stoichiometrically and with the same time course. The reaction is inhibited by DPN; hence it may be inferred that enzyme-adenylate… Expand

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