Enzymatic Incorporation of LNA Nucleotides into DNA Strands

@article{Veedu2007EnzymaticIO,
  title={Enzymatic Incorporation of LNA Nucleotides into DNA Strands},
  author={Rakesh N. Veedu and Birte Vester and Jesper Wengel},
  journal={ChemBioChem},
  year={2007},
  volume={8}
}
Unlocking uses of locked nucleic acids: LNA nucleoside 5′-triphosphates have been synthesized, and their ability to serve as substrates for polymerases have been investigated. Phusion high-fidelity DNA polymerase was found to be an efficient enzyme for incorporating LNA nucleoside 5′-triphosphates into DNA strands. 

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TLDR
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TLDR
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TLDR
The enzymatic incorporation of a series of emissive pyrimidine analogues into RNA oligonucleotides is explored and the 5-modified non-natural UTP is found to be the most "polymerase-friendly" and well accommodated by T7 RNA polymerase.

Enzymatic Synthesis of 7',5'-Bicyclo-DNA Oligonucleotides.

TLDR
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