Enzymatic (R)-phenylacetylcarbinol production in a benzaldehyde emulsion system with Candida utilis cells

@article{Satianegara2005EnzymaticP,
  title={Enzymatic (R)-phenylacetylcarbinol production in a benzaldehyde emulsion system with Candida utilis cells},
  author={Gernalia Satianegara and Michael Breuer and Bernhard Hauer and Peter L. Rogers and Bettina Rosche},
  journal={Applied Microbiology and Biotechnology},
  year={2005},
  volume={70},
  pages={170-175}
}
Recent progress in enzymatic (R)-phenylacetylcarbinol (PAC) production has established the need for low cost and efficient biocatalyst preparation. Pyruvate decarboxylase (PDC) added in the form of Candida utilis cells showed higher stability towards benzaldehyde and temperature in comparison with partially purified preparations. In the presence of 50 mM benzaldehyde and at 4°C, a half-life of 228 h was estimated for PDC added as C. utilis cells, in comparison with 24 h for the partially… CONTINUE READING