Environmental conditions which influence mucoid conversion Pseudomonas aeruginosa PAO1

@article{Terry1991EnvironmentalCW,
  title={Environmental conditions which influence mucoid conversion Pseudomonas aeruginosa PAO1},
  author={J. M. Terry and S. E. Pi{\~n}a and S. Mattingly},
  journal={Infection and Immunity},
  year={1991},
  volume={59},
  pages={471 - 477}
}
Growth and conversion to the mucoid phenotype by nonmucoid Pseudomonas aeruginosa PAO1 was studied in a chemostat system under conditions designed to reflect those likely to be present during chronic infection in the lung in cystic fibrosis patients. Mucoid variants were consistently isolated during continuous culture in the presence of 0.3 M NaCl or 5 or 10% glycerol. Mucoid subpopulations were also detected under conditions of carbon, nitrogen, or phosphate limitation. During carbon or… Expand
Role of energy metabolism in conversion of nonmucoid Pseudomonas aeruginosa to the mucoid phenotype
TLDR
A significant role for the energy state of the cell in conversion toMucoid and in selection for the mucoid phenotype is demonstrated. Expand
Vanadate and triclosan synergistically induce alginate production by Pseudomonas aeruginosa strain PAO1
TLDR
The addition of ammonium metavanadate (AMV), a phosphatase inhibitor, to PIA (PIA‐AMV) induced mucoidy in both these laboratory strains and early lung colonizing non‐mucoid isolates with a wt mucA, suggesting a model of alginate induction and the PIA‐AMI medium may be suitable for examining early lung colonization phenotypes in CF before the selection of the mucA mutants. Expand
Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors
The conversion of Pseudomonas aeruginosa into the mucoid, exopolysaccharide alginate-overproducing form is considered to be a major pathogenic determinant expressed by this organism during chronicExpand
Evidence for Sigma Factor Competition in the Regulation of Alginate Production by Pseudomonas aeruginosa
TLDR
The results indicate that the anti-σ70 factors can induce conversion to mucoidy in P. aeruginosa CF149 with algU-suppressor mutation via modulation of RpoD. Expand
Differentiation of Pseudomonas aeruginosa into the alginate‐producing form: inactivation of mucB causes conversion to mucoidy
TLDR
A loss of mucB function is sufficient to cause conversion of P. aeruginosa into the mucoid phenotype, and the complete nucleotide sequence of mucA and mucH was determined. Expand
Impact of glycerol-3-phosphate dehydrogenase on virulence factor production by Pseudomonas aeruginosa.
TLDR
This study shows that some CF-adapted P. aeruginosa isolates utilize glycerol more efficiently as a carbon source than nonadapted isolates, and suggests that interference with this metabolic pathway may have potential therapeutic benefits. Expand
Genetic rearrangement associated with in vivo mucoid conversion of Pseudomonas aeruginosa PAO is due to insertion elements
TLDR
The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats and this type of genetic recombination may also be associated with mucoids conversion in some P. aerug inosa clinical isolates. Expand
Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems.
TLDR
Results suggest that surfaces in aquatic environments may represent a natural habitat for mucoid (i.e. alginate-overproducing) strains of Ps. Expand
Energy metabolism and alginate biosynthesis in Pseudomonas aeruginosa: role of the tricarboxylic acid cycle
TLDR
It is shown that the levels of phosphorylated succinyl coenzyme A synthetase (Scs) and nucleoside diphosphate kinase (Ndk), which form a complex in P. aeruginosa, are reduced in the algR2 mutant, which indicates that AlgR 2 is responsible for maintaining proper operation of the TCA cycle and energy metabolism. Expand
La biosynthèse des alginates chez Pseudomonas aeruginosa: Régulation de l'expression d'un facteur de virulence
Summary Alginate is an important virulence factor in Pseudomonas aeruginosa, an ubiquitous bacterium which produces this particular exopolysaccharide almost exclusively in association with chronicExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 37 REFERENCES
Aeration selects for mucoid phenotype of Pseudomonas aeruginosa
TLDR
It is suggested that nonmucoid revertants may have an unusual sensitivity to aeration, which may indicate a mechanism for natural selection of the mucoid phenotype in vivo. Expand
Phosphorylcholine stimulates capsule formation of phosphate-limited mucoid Pseudomonas aeruginosa
TLDR
Electron micrographs showed the gradual formation of a capsule during growth on phosphorylcholine, indicating that the mucoid strain has the ability to utilize surfactant not only as a nutrient source but also for constructing a capsule with greatly enhanced adhesive properties. Expand
High osmolarity is a signal for enhanced algD transcription in mucoid and nonmucoid Pseudomonas aeruginosa strains
TLDR
In both mucoid and nonmucoid P. aeruginosa strains (containing a functional algR gene), transcriptional activation of algD increased as the osmolarity of the culture medium increased, suggesting that other environmental factors are involved in full activation of the alginate genes. Expand
Quantitation of adherence of mucoid and nonmucoid Pseudomonas aeruginosa to hamster tracheal epithelium
TLDR
Adherence of mucoid and nonmucoid isolates of Pseudomonas aeruginosa to tracheal epithelium was quantitated by using hamster tracheas to determine the mechanism of adherence of the bacteria to the ciliated epithelia of the respiratory tract. Expand
Production and Characterization of the Slime Polysaccharide of Pseudomonas aeruginosa
TLDR
The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated and were found to be similar to each other, to the slime of Azotobacter vinelandii, and to seaweed alginic acids. Expand
Role of Pseudomonas aeruginosa mucoid exopolysaccharide in adherence to tracheal cells
TLDR
The data strongly support the thesis that the mucoid exopolysaccharide is the adhesion forMucoid strains of P. aeruginosa, by measuring the binding of 14C-labeled mucoids to injured tracheas and testing whether an antibody against the major epitope of themucoidExopolysac charide inhibits adherence of these organisms. Expand
Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa
TLDR
In batch cultures of Pseudomonas aeruginosa, the maximum rate of exopolysaccharide synthesis occurred during exponential growth and nonmucoid variants appeared after seven generations in continuous culture and rapidly increased in proportion to the total number of organisms present. Expand
The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes
TLDR
DNA sequence analysis of algR revealed the homology of its gene product with a recently recognized class of environmentally responsive bacterial regulatory genes, including ompR, phoB, sfrA, ntrC, spoOA, dctD, and virG, which indicate that novel conditions in lungs affected by cystic fibrosis may be participating in the control of mucoidy. Expand
Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate
TLDR
Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis commonly produce a capsule-like exopolysaccharide called alginate, which results in a mucoid colony morphology and is an unstable trait. Expand
Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa
TLDR
Mucoid strains of Pseudomonas aeruginosa isolated from the sputum of cystic fibrosis patients produce copious quantities of an exopolysaccharide known as alginic acid, which has been difficult to isolate individual structural gene mutants defective in alginate synthesis. Expand
...
1
2
3
4
...