Environmental DNA metabarcoding of wild flowers reveals diverse communities of terrestrial arthropods

  title={Environmental DNA metabarcoding of wild flowers reveals diverse communities of terrestrial arthropods},
  author={Philip Francis Thomsen and Eva Egelyng Sigsgaard},
  journal={Ecology and Evolution},
  pages={1665 - 1679}
Abstract Terrestrial arthropods comprise the most species‐rich communities on Earth, and grassland flowers provide resources for hundreds of thousands of arthropod species. Diverse grassland ecosystems worldwide are threatened by various types of environmental change, which has led to decline in arthropod diversity. At the same time, monitoring grassland arthropod diversity is time‐consuming and strictly dependent on declining taxonomic expertise. Environmental DNA (eDNA) metabarcoding of… 

Environmental DNA metabarcoding of cow dung reveals taxonomic and functional diversity of invertebrate assemblages

This study investigates the potential of environmental DNA (eDNA) metabarcoding of cow dung samples for biomonitoring of dung‐associated invertebrates and discusses potential caveats of the method, as well as directions for future study and perspectives for implementation in research and monitoring.

Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding

Test multiple sample substrates (soil, scat, plant material and bulk arthropods) to determine what organisms can be detected from each and where they overlap demonstrate the importance of selecting appropriate metabarcoding substrates when undertaking terrestrial surveys.

Assessing flower-visiting arthropod diversity in apple orchards through environmental DNA flower metabarcoding and visual census

Arthropods are essential to maintaining healthy and productive agricultural systems. Apples are cultivated worldwide and rely on pollination. Honey bees are used for pollination but wild bees and

Environmental DNA from archived leaves reveals widespread temporal turnover and biotic homogenization in forest arthropod communities

A major limitation of current reports on insect declines is the lack of standardized, long-term, and taxonomically broad time series. Here, we demonstrate the utility of environmental DNA from

Airborne environmental DNA metabarcoding for the monitoring of terrestrial insects - a proof of concept

It is suggested that airborne eDNA has the potential to become a powerful tool for terrestrial biodiversity monitoring, with many impactful applications including the monitoring of pests, invasive or endangered species or disease vectors.

Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity

Simple Summary The innovative concept of environmental DNA has found its foot in aquatic ecosystems but remains an unexplored area of research concerning terrestrial ecosystems. When making

BeeDNA: microfluidic environmental DNA metabarcoding as a tool for connecting plant and pollinator communities

Microfluidic eDNA metabarcoding requires optimization but shows promise in revealing complex networks underpinning critical ecosystem functions and services, enabling more accurate assessments of ecosystem resilience.

Evaluating restoration trajectories using DNA metabarcoding of ground‐dwelling and airborne invertebrates and associated plant communities

Invertebrates are important for restoration processes as they are key drivers of many landscape‐scale ecosystem functions; including pollination, nutrient cycling and soil formation. However,

A molecular method for biomonitoring of an exotic plant‐pest: Leafmining for environmental DNA

A robust method based on environmental DNA (eDNA) was developed exploiting traces of DNA left inside 'empty' leaf mines, which are straightforward to collect and persist longer in the environment than the fly, extending the window of possible diagnosis to at least 28 days after a leaf mine becomes empty.

DNA barcoding of insects from India: Current status and future perspectives

The present paper describes the current status of barcoding of insect species in India along with the gaps that need to be remedied and shows that barcoded specimens cover a very meagre proportion of less than 3.73% of the known taxa/described species.



Metabarcoding and mitochondrial metagenomics of endogean arthropods to unveil the mesofauna of the soil

The FBF protocol together with the PCR‐based and shotgun sequencing pipelines addressed most of the challenges of studying soil arthropod mesofauna on the MiSeq Illumina platform and are powerful, cost‐efficient tools for characterising soil diversity in a phylogenetic and community ecology context.

How many species of arthropods visit flowers?

  • C. Wardhaugh
  • Environmental Science
    Arthropod-Plant Interactions
  • 2015
An overview of the taxonomic range of flower-visiting invertebrates is presented and it is speculated, based on reviewing the literature and discussions with experts, that ~30 % of arthropod species may regularly utilise flowers to feed, find a mate, or acquire other resources.

Using metabarcoding to reveal and quantify plant-pollinator interactions

If metabarcoding may circumvent the limits of conventional methodologies in detecting and quantifying plant-pollinator interactions, it holds great promise for investigating diverse facets of interactions and will provide a new perception of pollination networks as a whole.

Mitochondrial metagenomics: letting the genes out of the bottle

Mitochondrial metagenomics offers a promising avenue for unifying the ecological and evolutionary understanding of species diversity and makes it possible to obtain data on spatial and temporal turnover in whole-community phylogenetic and species composition, even in complex ecosystems where species-level taxonomy and biodiversity patterns are poorly known.

Meta‐barcoding of ‘dirt’ DNA from soil reflects vertebrate biodiversity

It is found that DNA from the soil surface reflects overall taxonomic richness and relative biomass of individual species, however, one species that was recently introduced was not detected and animal behaviour was shown to influence DNA deposition rates.

Monitoring endangered freshwater biodiversity using environmental DNA.

It is demonstrated that entire faunas of amphibians and fish can be detected by high-throughput sequencing of DNA extracted from pond water, underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for monitoring rare and threatened species across a wide range of taxonomic groups.

DNA barcoding insect–host plant associations

It is demonstrated that plant barcoding is already feasible with the current publicly available data and strong physical evidence for the host association is provided by sequencing plant barcodes directly from DNA extractions made from herbivorous beetles.

Applying pollen DNA metabarcoding to the study of plant–pollinator interactions1

A proof-of-concept quantitative pollination network using pollen metabarcoding has advantages in efficiency and resolution over microscopic identification of pollen, and it is expected that it will have broad utility for future studies of plant–pollinator interactions.

Uncovering Trophic Interactions in Arthropod Predators through DNA Shotgun-Sequencing of Gut Contents

Good taxonomic breadth and resolution was achieved (93% of prey identified to species or genus), but with low recovery of matching reads, and caveats and some future prospects that could improve the use of direct DNA shotgun-sequencing to characterize arthropod trophic networks are discussed.

The ecologist's field guide to sequence‐based identification of biodiversity

The aim here is to succinctly describe the different technologies available within the omics toolbox and showcase the opportunities available to contemporary ecologists to advance the understanding of biodiversity and its potential roles in ecosystems.