Environment-dependent mechanism of dehalogenation by Rhodococcus chlorophenolicus PCP-1

Abstract

Cells and cell-free preparations of a soil-bioremediating organism, Rhodococcus chlorophenolicus PCP-1, dehalogenated polychlorophenols both under aerobic and anaerobic conditions. Under aerobic conditions molecular O2 served as the source of oxygen for the dechlorinating para-hydroxylation reaction. Chlorophenols were dehalogenated and para-hydroxylated also under anaerobic conditions by a cyt P-450 enzyme. Water was used anaerobically as an oxygen source but the reaction required the presence of sulphite ions or iodosobenzene. When the dehalogenating enzyme was given a choice between molecular O2 and water in the presence of sulphite ions or iodosobenzene, the oxygen was preferably derived from water.

DOI: 10.1007/BF00170095

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Cite this paper

@article{Uotila2004EnvironmentdependentMO, title={Environment-dependent mechanism of dehalogenation by Rhodococcus chlorophenolicus PCP-1}, author={Jussi S. Uotila and Veikko H. Kitunen and Juha H. A. Apajalahti and Mirja S. Salkinoja-Salonen}, journal={Applied Microbiology and Biotechnology}, year={2004}, volume={38}, pages={408-412} }