Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells.

Envelope-associated nucleoids have been isolated from Caulobacter crescentus by using a modification of the procedure of T. Kornberg et al. (Proc. Natl. Acad. Sci. U.S.A. 71:3189-3193, 1974). The development of a Ludox density gradient procedure has permitted preparation of large quantities of synchronous cells. The sedimentation coefficients of the… CONTINUE READING