EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro.

Abstract

By fusing the transcriptional and translational start signals of lacZ to envZ, we have obtained high-level synthesis of a truncated EnvZ protein (EnvZ115) in which the first 38 amino acids of EnvZ are replaced with the first 8 amino acids of LacZ. Using this construct, we have partially purified the EnvZ115 protein and demonstrated that this protein can be… (More)

4 Figures and Tables

Topics

  • Presentations referencing similar topics