Enumeration of viable Escherichia coli by real-time PCR with propidium monoazide.

Abstract

A photo-inducible DNA-binding dye, propidium monoazide (PMA), was used to distinguish viable and dead Escherichia coli cells. Microscopic observations using a combination of the dyes 4',6-diamidino-2-phenylindole and PMA indicated that PMA stained only dead cells, with membrane damage, red. Mixtures of viable and heat-treated E. coli cells were subjected to… (More)
DOI: 10.2166/wst.2012.370

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