Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies.

  title={Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies.},
  author={C. Alanio and F. Lemaı̂tre and H. Law and M. Hasan and M. Albert},
  volume={115 18},
  • C. Alanio, F. Lemaı̂tre, +2 authors M. Albert
  • Published 2010
  • Biology, Medicine
  • Blood
  • The number of antigen-specific naive CD8(+) T cells is believed to be important in the shaping of adaptive immune responses, and is predictive for the magnitude of priming responses in mouse models. Because of extremely low precursor frequencies, knowledge about these cells comes from indirect techniques and estimations. Here, we present a strategy based on the combination of tetramer staining, magnetic-bead enrichment, and multiparametric cytometry, which permitted direct detection and… CONTINUE READING
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