Enumeration of Methanobrevibacter smithii in human feces

Abstract

A platin medium containing cephalothin and clindamycin was developed for enumeration and isolation of methanogens in human feces. Specimens from nine CH4-producing subjects had total anaerobe counts of 1–8×1011/g dry weight. Methanogen counts on the antibiotic medium ranged from 0.001–12.6% of the total anaerobe count. There was no correlation between age, sex, or percent dry fecal weight and the ratio of methanogens to total counts. Specimens from eight non-CH4-producing individuals contained bacteria thay yielded nonmethanogenic colonies on the antibiotic medium. The means±SD of the logarithm of the total counts per gram dry weight were 11.4±0.29 and 11.38±0.44 for the positive and negative groups respectively. Values for the antibiotic-resistant flora were 8.8±1.13 and 7.78±1.08 respectively. Methanogens were isolated from the most dilute inoculum of each specimen from CH4-producing subjects. All isolates were morphologically, physiologically, and immunologically identical to Methanobrevibacter smithii. Growth of methanogens in media that were essentially extracts of CH4-negative feces suggested that no nutrients were lacking or inhibitors present in intestinal contents that prevent the growth of methanogens in these individuals.

DOI: 10.1007/BF00451492

5 Figures and Tables

0100200'85'88'91'94'97'00'03'06'09'12'15
Citations per Year

1,048 Citations

Semantic Scholar estimates that this publication has 1,048 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Miller1982EnumerationOM, title={Enumeration of Methanobrevibacter smithii in human feces}, author={Terry L. Miller and Meyer J. Wolin}, journal={Archives of Microbiology}, year={1982}, volume={131}, pages={14-18} }