Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay.

@article{Ouwerkerk2002EnumerationOM,
  title={Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay.},
  author={D Ouwerkerk and A V Klieve and Robert J. Forster},
  journal={Journal of applied microbiology},
  year={2002},
  volume={92 4},
  pages={753-8}
}
AIMS To develop a real-time Taq nuclease assay (TNA) to enable the in vivo enumeration of Megasphaera elsdenii. METHODS AND RESULTS Megasphaera elsdenii YE34 was phenotypically characteristic of the species and had 16S rDNA sequence similarity of 98% to previously described isolates. Calibration of the number of cells of M. elsdenii against the cycle threshold of fluorescent dye release gave a straight-line relationship with a correlation coefficient approximating unity. The specificity of… CONTINUE READING

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