Enucleation by centrifugation of in vitro-matured bovine oocytes for use in nuclear transfer.

@article{Tatham1995EnucleationBC,
  title={Enucleation by centrifugation of in vitro-matured bovine oocytes for use in nuclear transfer.},
  author={Brendan G Tatham and A T Dowsing and Alan O. Trounson},
  journal={Biology of reproduction},
  year={1995},
  volume={53 5},
  pages={1088-94}
}
Nuclear transfer has the potential to produce large numbers of identical progeny. Current limitations of the technique are associated with the use of micromanipulation for the demanding enucleation and reconstitution procedure. With the overcoming of this limitation, increased numbers of nuclear transfer embryos could be produced. Centrifugation of bovine oocytes at 15,000 x g for 2 min resulted in the stratification of organelles within the cytoplasm which positioned the metaphase II spindle… CONTINUE READING