Entrapping of impermeant probes of different size into nonpermeabilized synaptosomes as a method to study presynaptic mechanisms.

@article{Raiteri2000EntrappingOI,
  title={Entrapping of impermeant probes of different size into nonpermeabilized synaptosomes as a method to study presynaptic mechanisms.},
  author={Maurizio Raiteri and Roser Sala and Anna Fassio and Ornella Rossetto and Giambattista Bonanno},
  journal={Journal of neurochemistry},
  year={2000},
  volume={74 1},
  pages={423-31}
}
Small molecules present during brain tissue homogenization are known to be entrapped within subsequently isolated synaptosomes. We have revisited this technique in view of its systematic utilization to incorporate into nerve endings impermeant probes of large size. Rat neocortical synaptosomes were prepared in the absence or in the presence of each of the… CONTINUE READING