Entrainment of heterogeneous glycolytic oscillations in single cells

  title={Entrainment of heterogeneous glycolytic oscillations in single cells},
  author={Anna-Karin Gustavsson and Caroline Beck Adiels and Bernhard Mehlig and Mattias Goks{\"o}r},
  journal={Scientific Reports},
Cell signaling, gene expression, and metabolism are affected by cell-cell heterogeneity and random changes in the environment. The effects of such fluctuations on cell signaling and gene expression have recently been studied intensively using single-cell experiments. In metabolism heterogeneity may be particularly important because it may affect synchronisation of metabolic oscillations, an important example of cell-cell communication. This synchronisation is notoriously difficult to describe… 

Synchronisation of glycolytic activity in yeast cells

The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast, a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold.

Synchronisation of glycolytic activity in yeast cells

The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast, a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold.

Partial synchronisation of glycolytic oscillations in yeast cell populations

The transition between synchronized and asynchronous behaviour of immobilized yeast cells of the strain Saccharomyces carlsbergensis was investigated by monitoring the autofluorescence of the coenzyme NADH, and it was suggested that synchronisation occurred due to entrainment by the cells that oscillated more rapidly.

Phosphofructokinase controls the acetaldehyde-induced phase shift in isolated yeast glycolytic oscillators.

It is found that the model quantitatively predicts the phase-dependent phase shift observed in the experimental data and establishes that phosphofructokinase plays a key role in the phase shift dynamics as shown in the PRC and adaptation time to external perturbations.

Thesis for the degree of Doctor of Philosophy Metabolic communication between individual yeast cells

The design, simulation and fabrication of microfluidic systems that allowed for the environmental control and experimental observation of glycolytic synchronization between individual yeast cells are reported, and it is shown that synchronization communities are formed depending on the exposure ratios of cyanide and glucose, and the exchanged acetaldehyde.

Emergent oscillations in dense adaptive cell populations

It is shown that a sufficient condition for oscillations is fulfilled by adaptive cells which reset their signal secretion rate upon prolonged stimulation, and a quantitative theory for the phenomenon is developed and present a necessary condition for collective oscillations in a communicating population.

Intercellular communication induces glycolytic synchronization waves between individually oscillating cells

It is shown that cells coupled via diffusion of metabolites can lead to spatially differentiated subpopulations with temporally synchronized behavior, and how a relatively simple mechanism can underlie a yet poorly understood cellular differentiation process is indicated.

Studying Glycolytic Oscillations in Individual Yeast Cells by Combining Fluorescence Microscopy with Microfluidics and Optical Tweezers

This unit presents a step‐by‐step guide for fabrication of the microfluidic device, for alignment of the optical tweezers, for cell preparation, and for time‐lapse imaging of glycolytic oscillations in single cells, including a discussion of common pitfalls.

Entrainment of a Bacterial Synthetic Gene Oscillator through Proteolytic Queueing.

This work studies the entrainment of gene networks by a queueing interaction established by competition between proteins for a common proteolytic pathway in Escherichia coli through pulsatile production of an additional protein competing for acommon degradation pathway with the oscillator proteins.

Effect of the periodic properties of toxic stress on the oscillatory behaviour of glycolysis in yeast-evidence of a toxic effect frequency.

  • C. AndréF. Gagné
  • Biology
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
  • 2017



Effect of cellular interaction on glycolytic oscillations in yeast: a theoretical investigation.

The oscillatory dynamics of synchronized cell populations are investigated by calculating the phase responses to acetaldehyde pulses, and it is concluded that addition to the transmembrane exchange of acetaldehyde, other processes may contribute to intercellular coupling, or that intracellular regulator feedback plays a role in the acceleration of the synchronization.

Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells

The experiments were analyzed in a detailed mathematical model and could be interpreted in terms of an oscillatory manifold in a three‐dimensional state‐space; crossing the boundaries of the manifold coincides with the onset of oscillations and positioning along the longitudinal axis of the volume sets the period.

Sustained glycolytic oscillations in individual isolated yeast cells

This is the first time that sustained limit‐cycle oscillations have been demonstrated in isolated yeast cells, and a detailed kinetic model for the cellular reactions is used, assuming small differences in a single internal parameter.

Stochasticity of metabolism and growth at the single-cell level

Time-lapse microscopy is used to measure fluctuations in the instantaneous growth rate of single cells of Escherichia coli, and quantify time-resolved cross-correlations with the expression of lac genes and enzymes in central metabolism to suggest that cellular metabolism is inherently stochastic, and a generic source of phenotypic heterogeneity.

Cell density dependence of oscillatory metabolism

Modifications in the metabolic behaviour of intact cell suspensions achieved by manipulating the strength of their interaction through changes in cell density are described.

Quantitative characterization of cell synchronization in yeast

This work finds that acetaldehyde has a very strong synchronization effect that can account quantitatively for the classical mixing experiment, and shows that glucose can mediate this kind of synchronization, provided that the glucose transporter is not saturated.

Synchrony and mutual stimulation of yeast cells during fast glycolytic oscillations

Modelling studies and experimental analysis of the kinetics of damping of oscillations and their amplitude suggested that the autonomous glycolytic oscillations of the yeast cells depend upon the balance between oxidative and reductive fluxes of NADH, which is affected by the extracellular concentration of ethanol.

Synchronization of glycolytic oscillations in a yeast cell population.

It is shown how this system in a CSTR (continuous flow stirred tank reactor) can be modelled quantitatively as a population of Stuart-Landau oscillators interacting by exchange of metabolites through the extracellular medium, thus reducing the complexity of the problem without sacrificing the biochemical realism.