Entrainment of heterogeneous glycolytic oscillations in single cells

@article{Gustavsson2015EntrainmentOH,
  title={Entrainment of heterogeneous glycolytic oscillations in single cells},
  author={Anna-Karin Gustavsson and Caroline Beck Adiels and Bernhard Mehlig and Mattias Goks{\"o}r},
  journal={Scientific Reports},
  year={2015},
  volume={5}
}
Cell signaling, gene expression, and metabolism are affected by cell-cell heterogeneity and random changes in the environment. The effects of such fluctuations on cell signaling and gene expression have recently been studied intensively using single-cell experiments. In metabolism heterogeneity may be particularly important because it may affect synchronisation of metabolic oscillations, an important example of cell-cell communication. This synchronisation is notoriously difficult to describe… 

Synchronisation of glycolytic activity in yeast cells

The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast, a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold.

Synchronisation of glycolytic activity in yeast cells

The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast, a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold.

Partial synchronisation of glycolytic oscillations in yeast cell populations

The transition between synchronized and asynchronous behaviour of immobilized yeast cells of the strain Saccharomyces carlsbergensis was investigated by monitoring the autofluorescence of the coenzyme NADH, and it was suggested that synchronisation occurred due to entrainment by the cells that oscillated more rapidly.

Phosphofructokinase controls the acetaldehyde-induced phase shift in isolated yeast glycolytic oscillators.

It is found that the model quantitatively predicts the phase-dependent phase shift observed in the experimental data and establishes that phosphofructokinase plays a key role in the phase shift dynamics as shown in the PRC and adaptation time to external perturbations.

Thesis for the degree of Doctor of Philosophy Metabolic communication between individual yeast cells

The design, simulation and fabrication of microfluidic systems that allowed for the environmental control and experimental observation of glycolytic synchronization between individual yeast cells are reported, and it is shown that synchronization communities are formed depending on the exposure ratios of cyanide and glucose, and the exchanged acetaldehyde.

Emergent oscillations in dense adaptive cell populations

It is shown that a sufficient condition for oscillations is fulfilled by adaptive cells which reset their signal secretion rate upon prolonged stimulation, and a quantitative theory for the phenomenon is developed and present a necessary condition for collective oscillations in a communicating population.

Intercellular communication induces glycolytic synchronization waves between individually oscillating cells

It is shown that cells coupled via diffusion of metabolites can lead to spatially differentiated subpopulations with temporally synchronized behavior, and how a relatively simple mechanism can underlie a yet poorly understood cellular differentiation process is indicated.

Studying Glycolytic Oscillations in Individual Yeast Cells by Combining Fluorescence Microscopy with Microfluidics and Optical Tweezers

This unit presents a step‐by‐step guide for fabrication of the microfluidic device, for alignment of the optical tweezers, for cell preparation, and for time‐lapse imaging of glycolytic oscillations in single cells, including a discussion of common pitfalls.

Entrainment of a Bacterial Synthetic Gene Oscillator through Proteolytic Queueing.

This work studies the entrainment of gene networks by a queueing interaction established by competition between proteins for a common proteolytic pathway in Escherichia coli through pulsatile production of an additional protein competing for acommon degradation pathway with the oscillator proteins.

Effect of the periodic properties of toxic stress on the oscillatory behaviour of glycolysis in yeast-evidence of a toxic effect frequency.

  • C. AndréF. Gagné
  • Biology
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
  • 2017

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