Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts

  title={Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts},
  author={Y. Itoh and Y. Sugita‐Konishi and F. Kasuga and M. Iwaki and Y. Hara-Kudo and N. Saito and Y. Noguchi and H. Konuma and S. Kumagai},
  journal={Applied and Environmental Microbiology},
  pages={1532 - 1535}
ABSTRACT Using cultivation, immunofluorescence microscopy, and scanning electron microscopy, we demonstrated the presence of viable enterohemorrhagic Escherichia coli O157:H7 not only on the outer surfaces but also in the inner tissues and stomata of cotyledons of radish sprouts grown from seeds experimentally contaminated with the bacterium. HgCl2 treatment of the outer surface of the hypocotyl did not kill the contaminating bacteria, which emphasized the importance of either using seeds free… Expand
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