Corpus ID: 27114189

Enterococcus Faecalis Biofilm. Formation and Development in Vitro Observed by Scanning Electron Microscopy.

@article{Bulacio2015EnterococcusFB,
  title={Enterococcus Faecalis Biofilm. Formation and Development in Vitro Observed by Scanning Electron Microscopy.},
  author={M. A. Bulacio and Lucas R Galv{\'a}n and C. Gaudioso and R. Cangemi and M. Erimbaue},
  journal={Acta odontologica latinoamericana : AOL},
  year={2015},
  volume={28 3},
  pages={
          210-4
        }
}
Biofilm produced by Enterococcus faecalis isolated from root canals was detected by growing it on microplates and using 10% crystal violet stain, elution with alcohol and three procedures: no fixation, heat fixation and 10% formaldehyde fixation. The biofilm was evaluated using a Versamax Microplate Reader (USA). Twenty sterile root portions were incubated in TS broth with E. faecalis (108) for 48 hours, 4, 7, 14 and 30 days, after which they were processed and observed by scanning electron… Expand
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