Entering the era of single-cell transcriptomics in biology and medicine

@article{Sandberg2013EnteringTE,
  title={Entering the era of single-cell transcriptomics in biology and medicine},
  author={Rickard Sandberg},
  journal={Nature Methods},
  year={2013},
  volume={11},
  pages={22-24}
}
challenges in single-cell transcriptomics Currently available single-cell RNA-seq methods were developed with several different objectives. Full-length transcripts can be profiled, such that sequence reads cover the entire gene to quantify both gene and transcript isoforms and also monitor single-nucleotide polymorphisms and mutations9,11. In contrast, tag-based sequencing of 5ʹ or 3ʹ ends10,13 provides only an estimate of transcript abundance at the cost of coverage across gene structures but… CONTINUE READING