OBJECTIVE To select the best type of macroporous resin for enriching gentiopicroside from the leaves of Gentiana macropylla, and study its optimal conditions and parameters. METHODS Adsorption and desorption characteristics of gentiopieroside were carried out on the resins. HPLC was used to determine the content of gentiopieroside. RESULTS The better resin D-101 was chosen, and the optimal parameters were obtained. CONCLUSION The process is feasible to enrich gentiopicroside from the leaves of Gentiana macropylla Pall.