Enrichment of non-synchronized cells in the G1, S and G2 phases of the cell cycle for the study of apoptosis.

@article{Coquelle2006EnrichmentON,
  title={Enrichment of non-synchronized cells in the G1, S and G2 phases of the cell cycle for the study of apoptosis.},
  author={Arnaud Coquelle and Shahul Mouhamad and Marie O. Pequignot and Thorsten Braun and Gabrielle Carvalho and Sonia Vivet and Didier M{\'e}tivier and Maria Castedo and Guido Kroemer},
  journal={Biochemical pharmacology},
  year={2006},
  volume={72 11},
  pages={1396-404}
}
The susceptibility of cells to apoptosis induction is deeply influenced by their position in the cell cycle. Unfortunately, however, current methods for the enrichment of cells in defined phases of the cell cycle are mostly based on the synchronization of cells by agents or conditions that are intrinsically toxic and induce apoptosis on their own. We developed a novel procedure for the purification of cells in distinct phases of the cell cycle. This method is based on the stable transfection of… CONTINUE READING