Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography.

@article{Fountoulakis1999EnrichmentOL,
  title={Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography.},
  author={Michael Fountoulakis and M{\'a}ria Tak{\'a}cs and Peter Berndt and H. Hans Langen and Bal{\'a}zs Tak{\'a}cs},
  journal={Electrophoresis},
  year={1999},
  volume={20 11},
  pages={2181-95}
}
Visualization of low-copy-number gene products is essential for the detection of novel drug targets by differential protein expression studies. We investigated the enrichment of low-abundance proteins of Escherichia coli by hydroxyapatite chromatography. The proteins of the various pools collected from a ceramic hydroxyapatite column were analyzed by two-dimensional electrophoresis and identified by matrix-assisted laser desorption ionization mass spectrometry. Approximately 800 spots… CONTINUE READING