Enrichment of Mutations in Multiple DNA Sequences Using COLD-PCR in Emulsion

Abstract

BACKGROUND Multiplex detection of low-level mutant alleles in the presence of wild-type DNA would be useful for several fields of medicine including cancer, pre-natal diagnosis and infectious diseases. COLD-PCR is a recently developed method that enriches low-level mutations during PCR cycling, thus enhancing downstream detection without the need for… (More)
DOI: 10.1371/journal.pone.0051362

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@inproceedings{CastellanosRizaldos2012EnrichmentOM, title={Enrichment of Mutations in Multiple DNA Sequences Using COLD-PCR in Emulsion}, author={Elena Castellanos-Rizaldos and Coren A. Milbury and G. Mike Makrigiorgos}, booktitle={PloS one}, year={2012} }