Enrichment of Escherichia coli spheroplasts displaying scFv antibodies specific for antigens expressed on the human cell surface

@article{Qiu2010EnrichmentOE,
  title={Enrichment of Escherichia coli spheroplasts displaying scFv antibodies specific for antigens expressed on the human cell surface},
  author={Jun-Kang Qiu and Sang-Taek Jung and George D. Georgiou and Hai-Ying Hang},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={88},
  pages={1385-1391}
}
Anchored periplasmic expression (APEx) is a method for isolating high affinity ligand-binding proteins from large combinatorial libraries, and antibodies highly specific for soluble antigens were successfully isolated from APEx antibody libraries in combination with flow cytometric sorting (Harvey et al., Proc Natl Acad Sci USA 101(25):9193–9198, 2004). However, many disease markers and drug targets are localized on the cell surface, and often, unique posttranslational modifications and/or… CONTINUE READING
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