Enrichment of Antigen-specific B Lymphocytes by the Direct Removal of B Cells Not Bearing Specificity for the Antigen* by Sharyn M. Walker,$ G. C. Meinke,§ Any

  • WILLIAM O. WEIGLEII
  • Published 2003

Abstract

Antigen-specific B cells (ASC) 1 can be isolated from other lymphoid cells by their ability to specifically bind antigen. ASC were shown to bind to antigencoated glass bead columns by the inability of the passed cells to respond to that antigen (1, 2). Since attempts to elute the cells with excess antigen were unsuccessful (2), other means of obtaining the… (More)

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