Enrichment for Nicotiana heterokaryons after protoplast fusion and subsequent growth in agarose microdrops

@article{Thomas1988EnrichmentFN,
  title={Enrichment for Nicotiana heterokaryons after protoplast fusion and subsequent growth in agarose microdrops},
  author={Mark R. Thomas and Ray J. Rose},
  journal={Planta},
  year={1988},
  volume={175},
  pages={396-402}
}
Protoplasts isolated from Nicotiana tabacum L. leaves and Nicotiana suaveolens Lehm. cell suspensions have been fused with polyethylene glycol (PEG). Enrichment for heterokaryons was based on a Percoll flotation protocol which allowed a preparation with 50% heterokaryons to be obtained. The heterokaryons developed into calli whose hybrid nature was shown by polyacrylamide gel electrophoresis of esterase isoenzymes. Sensitivity of the mesophyll protoplasts to PEG and different buoyant densities… CONTINUE READING

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