Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule.

@article{Okada1991EnrichmentAC,
  title={Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule.},
  author={Shinichi Okada and Hiromitsu Nakauchi and Kazunari Nagayoshi and Shin-ichi Nishikawa and Yasusada Miura and Toshio Suda},
  journal={Blood},
  year={1991},
  volume={78 7},
  pages={
          1706-12
        }
}
The proto-oncogene c-kit encodes a transmembrane tyrosine kinase receptor for stem cell factor (SCF). The c-kit/SCF signal is expected to have an important role in hematopoiesis. A monoclonal antibody (ACK-2) against the murine c-kit molecule was prepared. Flow cytometric analysis showed that the bone marrow cells that expressed the c-kit molecule (approximately 5%) were B220(B)-, TER119(erythroid)-, Thy1negative-low, and WGA+. A small number of Mac-1(macrophage)+ or Gr-1(granulocyte)+ cells… CONTINUE READING

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