Enrichment and biochemical characterization of boundary membrane contact sites from rat-liver mitochondria.

@article{Ohlendieck1986EnrichmentAB,
  title={Enrichment and biochemical characterization of boundary membrane contact sites from rat-liver mitochondria.},
  author={Kay Ohlendieck and I. Riesinger and Volker Adams and Jessica Krause and Dieter G Brdiczka},
  journal={Biochimica et biophysica acta},
  year={1986},
  volume={860 3},
  pages={672-89}
}
A subfraction of mitochondrial membranes was prepared from osmotically lysed rat liver mitochondria by density gradient centrifugation which contained the inner boundary membrane and the contact sites between this membrane and the outer membrane. The fraction was composed of inner and outer limiting membrane components as shown by the presence of specific marker enzymes, monoamine oxidase and glycerolphosphate oxidase. Surface proteolysis analysis, studies of cytochrome c permeability, and… CONTINUE READING

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