Enriched autonomously replicating sequences in a nuclear matrix-DNA complex isolated from synchronized HeLa cells.


Nucleoids isolated from either synchronized or exponentially growing HeLa cells were digested with restriction enzymes to separate a nuclear matrix-bound DNA component from the rest. Partial libraries were constructed by inserting DNA fragments from both components into a yeast-bacteria plasmid vector. A random sample from these libraries was tested for ARS… (More)


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