Enhancing immune responses using suicidal DNA vaccines

@article{Berglund1998EnhancingIR,
  title={Enhancing immune responses using suicidal DNA vaccines},
  author={Peter Berglund and Cristian Smerdou and Marina N. Fleeton and loannis Tubulekas and Peter Liljestr{\"o}m},
  journal={Nature Biotechnology},
  year={1998},
  volume={16},
  pages={562-565}
}
We describe a DNA vaccine strategy that allows antigens to be produced in vivo in the context of an alphaviral replicon. Mice immunized with such vectors developed humoral and cellular immune responses at higher levels than mice that received a conventional DNA vaccine vector. Immunized animals acquired protective immunity to lethal influenza challenge. Compared with traditional DNA vaccine strategies in which vectors are persistent and the expression constitutive, the expression mediated by… 
Enhancement of suicidal DNA vaccine potency by linking Mycobacterium tuberculosis heat shock protein 70 to an antigen
TLDR
This fusion converted a less effective vaccine into one with significant potency against established E7-expressing metastatic tumors, and the antitumor effect was predominantly CD8-dependent.
Alphavirus vectors for vaccine production and gene therapy
TLDR
Systemic delivery of many viral antigens has elicited strong antibody responses in immunized mice and primates, and protection against challenges with lethal viruses was obtained, and prophylactic vaccination was established against tumor challenges.
Enhancement of tumor-specific immune response with plasmid DNA replicon vectors.
TLDR
Plasmid DNA replicons mediated antigen production that was homogeneous in all transfected cells and associated with the apoptotic death of the host cells and may be useful in the development of recombinant vaccines for infectious diseases and cancer.
Alphavirus Vectors in Vaccine Development
TLDR
The high-level heterologous gene expression provided by alphavirus vectors has accelerated their applications in vaccine development, and novel approaches include the application of RNA interference and microRNA.
Alphavirus replicon-based strategies for vaccination
TLDR
The use of alphavirus replicons as a vaccine platform is evaluated and antigen-specific immune responses induced in mice and rhesus macaques are characterized, showing that DREP is an excellent prime of T cell and antibody responses prior to a heterologous boost immunization.
Delivery Systems for Gene-based Vaccines
TLDR
New approaches for formulating and delivering plasmid DNA and alphaviral replicon vectors, all of which have resulted in increased potency of gene-based vaccines are reviewed.
Genetic vaccination strategies for enhanced cellular, humoral and mucosal immunity
TLDR
The capacity to modulate and enhance specific immune responses by the use of approaches such as these may underpin the development of vaccines against diseases for which no effective strategies are currently available.
...
...

References

SHOWING 1-10 OF 37 REFERENCES
Induction of neonatal tolerance by plasmid DNA vaccination of mice.
TLDR
It is found that the same DNA vaccine induces tolerance rather than immunity when administered to 2-5 d-old mice, demonstrating important differences in the nature and specificity of the immune response elicited by DNA vaccines versus conventional protein immunogens.
Heterologous protection against influenza by injection of DNA encoding a viral protein.
TLDR
To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
DNA Immunization against Herpes Simplex Virus: Enhanced Efficacy Using a Sindbis Virus-Based Vector
TLDR
The in vivo efficacy of the Sindbis virus-based pSIN vectors as DNA vaccines and the potential usefulness of alphavirus-based vectors for DNA immunization in general and more specifically as a herpes simplex virus vaccine are demonstrated.
Protective cellular immunity: cytotoxic T-lymphocyte responses against dominant and recessive epitopes of influenza virus nucleoprotein induced by DNA immunization
TLDR
Mice immunized with wild-type or mutant NP DNA were protected from lethal cross-strain virus challenge, and the protection could be adoptively transferred by immune splenocytes, indicating the role of cell-mediated immunity in the protection.
Protective CTL-dependent immunity and enhanced immunopathology in mice immunized by particle bombardment with DNA encoding an internal virion protein.
TLDR
DNA vaccines are capable of inducing an anti-viral T cell response that can inhibit viral replication and mediate either protective immunity or immunopathology and may provide a useful alternative to current viral or subunit vaccines once the efficacy of immunization with DNA is optimized.
Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus.
TLDR
A plasmid vector, termed pSG5rab.gp, that expresses the rabies virus glycoprotein under the control of an SV40 early promoter, was tested in C3H/He mice for induction of rabie virus-specific immune responses and was fully protected against a subsequent challenge with rabiesirus.
A viral vaccine vector that expresses foreign genes in lymph nodes and protects against mucosal challenge
TLDR
Significant reduction of influenza virus replication in the nasal epithelia of HA vector-immunized mice suggested an effective immunity at the mucosal surface, suggesting that VEE vaccine vectors might be used successfully to protect against mucosal pathogens.
Genetic immunization is a simple method for eliciting an immune response
TLDR
It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
...
...