Enhancing functional expression of codon-optimized heterologous enzymes in Escherichia coli BL21(DE3) by selective introduction of synonymous rare codons.

@article{Zhong2017EnhancingFE,
  title={Enhancing functional expression of codon-optimized heterologous enzymes in Escherichia coli BL21(DE3) by selective introduction of synonymous rare codons.},
  author={Chao Zhong and Ping Wei and Yi-Heng Percival Zhang},
  journal={Biotechnology and bioengineering},
  year={2017},
  volume={114 5},
  pages={1054-1064}
}
Rare codon in a heterologous gene may cause premature termination of protein synthesis, misincorporation of amino acids, and/or slow translation of mRNA, decreasing the heterologous protein expression. However, its hypothetical function pertaining to functional protein folding has been barely reported. Here, we investigated the effects of selective… CONTINUE READING