Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP)

@article{Chong2014EnhancingEC,
  title={Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP)},
  author={Huiqing Chong and Hefang Geng and Hongfang Zhang and Hao Song and Lei Huang and Rongrong Jiang},
  journal={Biotechnology and Bioengineering},
  year={2014},
  volume={111}
}
The limited isobutanol tolerance of Escherichia coli is a major drawback during fermentative isobutanol production. Different from classical strain engineering approaches, this work was initiated to improve E. coli isobutanol tolerance from its transcriptional level by engineering its global transcription factor cAMP receptor protein (CRP). Random mutagenesis libraries were generated by error‐prone PCR of crp, and the libraries were subjected to isobutanol stress for selection. Variant IB2… Expand
Engineering of global regulator cAMP receptor protein (CRP) in Escherichia coli for improved lycopene production.
TLDR
This is the first report of improving the phenotype for metabolite overproduction in E. coli using a CRP engineering strategy, and the introduction of the mutant crp gene (mcrp26) increased β-carotene production in E coli. Expand
Enhancing succinic acid biosynthesis in Escherichia coli by engineering its global transcription factor, catabolite repressor/activator (Cra)
TLDR
It is suggested that some of the negative effect of FBP on Cra might have been counteracted through the enhanced binding affinity of the Cra mutant for FBP or the change of Cra structure, which provides useful information about understanding the transcriptional regulation of succinate biosynthesis. Expand
Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7
TLDR
This is the first study to demonstrate the possibility of engineering eukaryotic RNAP to alter global transcription profile and improve strain phenotypes and targeting subunit Rpb7 of RNAP II was able to bring differential expression in hundreds of genes in S. cerevisiae, which finally led to improvement in yeast ethanol tolerance and production. Expand
Engineering Synthetic Multistress Tolerance in Escherichia coli by Using a Deinococcal Response Regulator, DR1558
TLDR
The introduced deinococcal response regulator DR1558 was introduced into Escherichia coli, thereby conferring tolerance to hydrogen peroxide, and microarray analysis clearly showed that a variety of stress-responsive genes that are directly or indirectly controlled by RpoS were upregulated in strain Ec-1558. Expand
cAMP receptor protein (CRP)-mediated resistance/tolerance in bacteria: mechanism and utilization in biotechnology
TLDR
It is believed that transcriptional engineering of CRP can provide an alternative strain engineering method for E. coli and its detailed mechanism may need further investigations. Expand
Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB
TLDR
Saturation mutagenesis on T10 site revealed that hydrophobic residues were required for high butanol tolerance of E. coli, and SecB chaper one was identified by chaperone mining to be effective in enhancing butanol tolerated. Expand
Genome-wide Escherichia coli stress response and improved tolerance towards industrially relevant chemicals
TLDR
The combination of the transcriptional response and mutant screening provides general targets that can increase tolerance towards not only single, but multiple chemicals. Expand
Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli
TLDR
Enhanced binding of Cra to FBP or active site mutations may eliminate the repressive effect caused by FBP, thus leading to increased activation of genes associated with succinate biosynthesis in the Cra mutant. Expand
Tolerance against butanol stress by disrupting succinylglutamate desuccinylase in Escherichia coli
Background: The four-carbon alcohol, butanol, is emerging as a promising biofuel and efforts have been undertaken to improve several microbial hosts for its production. However, most organisms haveExpand
Natural Guided Genome Engineering Reveals Transcriptional Regulators Controlling Quorum-Sensing Signal Degradation
TLDR
It is demonstrated that QQ activity can be improved by modifying the regulation of QQ-enzymes degrading QS signal, which leads to an enhanced R. erythropolis in which the QS-signal degradation pathway is strongly activated. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 63 REFERENCES
Improving Ethanol Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP)
TLDR
To improve the ethanol tolerance of E. coli from the transcriptional level by engineering its global transcription factor cAMP receptor protein (CRP), which is known to regulate over 400 genes in E. bacteria, real-time reverse transcription PCR analysis (RT-PCR) revealed that 203 genes were differentially expressed in iE2 in the absence of ethanol, whereas 92 displayed differential expression when facing ethanol stress. Expand
Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance
TLDR
It is demonstrated that Escherichia coli 1-butanol tolerance can be greatly enhanced through random mutagenesis of global transcription factor cyclic AMP receptor protein (CRP) and direct manipulation of the transcript profile through engineering global transcription factors can provide a useful tool in strain engineering. Expand
Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance
TLDR
It is proved the concept that engineering global transcription factor cAMP receptor protein (CRP) of Escherichia coli can improve cell phenotypes by engineering strain osmotolerance is proved. Expand
Enhancing E. coli Tolerance towards Oxidative Stress via Engineering Its Global Regulator cAMP Receptor Protein (CRP)
TLDR
This is the first work on improving E. coli oxidative stress resistance by reframing its transcription machinery through its native global regulator cAMP receptor protein (CRP), which can directly or indirectly regulate redox-sensing regulators SoxR and OxyR, and other ∼400 genes inE. Expand
Engineering butanol‐tolerance in escherichia coli with artificial transcription factor libraries
TLDR
E. coli can be engineered as a promising host for high‐yield butanol production and identified genes of E. coli that are associated with the butanol‐tolerance. Expand
Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli
TLDR
The results suggest that C4, C5 alcohol stress impacts the cell differently compared with the general solvent or antibiotic stresses, and improved isobutanol tolerance did not increase the final titer of isOButanol production. Expand
An evolutionary strategy for isobutanol production strain development in Escherichia coli.
TLDR
A rapid evolutionary strategy for isolating strains of Escherichia coli that effectively produce isobutanol from glucose utilizing random mutagenesis and a growth selection scheme is developed. Expand
Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance
Abstract Organic solvents are often applied in biphasic biocatalysis or involved in bioremediation, but native microorganisms usually have low tolerance toward these organic solvents and cannotExpand
Improvement of organic solvent tolerance level of Escherichia coli by overexpression of stress-responsive genes
Abstract Water-immiscible organic solvents can be toxic to microorganisms. The tolerance levels differ among strains of Escherichia coli, suggesting that the organic solvent tolerance level is strainExpand
Functional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli
TLDR
Cell-wide studies were conducted at the transcript, protein, and metabolite levels to obtain a global view of the n-butanol stress response, allowing identification of key genes that were recruited to alleviate oxidative stress, protein misfolding, and other causes of growth defects. Expand
...
1
2
3
4
5
...