Enhancing DNA electrotransformation efficiency in Escherichia coli DH 10 B electrocompetent cells

@inproceedings{Wu2010EnhancingDE,
  title={Enhancing DNA electrotransformation efficiency in Escherichia coli DH 10 B electrocompetent cells},
  author={Ning Wu and Kanyand Matand and Bizuayehu Kebede and George Acquaah and Sonya Williams},
  year={2010}
}
Electrotransformation also known as electroporation is the most reliable and efficient tool for plasmid DNA uptake. Electrotransformation efficiency is function of many factors which include (1) number of cell washes prior to electroporation, (2) electroporation cell number, (3) electroporation DNA amount, and (4) cell growth phase. Those factors have limitedly been concomitantly investigated in E. coli DH10B strain. This study is aimed to explore above key factors to define the optimal… CONTINUE READING
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Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells

  • WU N., K. MATAND, B. KEBEDE, G. ACQUAAH, S. WILLIAMS
  • Electronic Journal of Biotechnology,
  • 2010

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